Análise epidemiológica das fraturas dos ossos da face em um hospital público no nordeste do Brasil / Análisis epidemiológico de las fracturas de los huesos de la cara en un hospital público del noreste de Brasil / Epidemiological analysis of face bone fractures in a public hospital in northeast Brazil

As fraturas dos ossos da região maxilofacial são ocorrências que podem se apresentar como quadros de urgência e/ou emergência na rotina das unidades de pronto atendimento e hospitais do mundo inteiro, principalmente em localidades com altos índices de violência interpessoal e infrações de trânsito. Um grande número de traumatismos na face, tanto em tecidos moles como duros acontece devido à enorme exposição e a pouca proteção desta região o que acarreta frequentemente lesões graves. Objetivo: O presente estudo tem como objetivo avaliar a prevalência das fraturas no complexo maxilofacial em uma unidade hospitalar pública, a partir de um estudo epidemiológico, sendo analisados os fatores relacionados a ocorrência do trauma, agente etiológico, distribuição das fraturas, gênero e idade dos indivíduos acometidos. Metodologia: O presente estudo do tipo transversal retrospectivo, onde foram avaliados 268 prontuários de pacientes diagnosticados com fraturas dos ossos da face atendidos no Hospital Regional Justino Luz, localizado na cidade de Picos, no estado do Piauí, Brasil, no período de janeiro de 2015 até janeiro de 2017, os prontuários foram analisados no setor de arquivo médico do HRJL. Resultados: os fatores etiológicos mais observados foram os acidentes motociclísticos, seguidos de agressão física e quedas da própria altura, o tipo de fratura mais comum foi a do Complexo Orbito-Zigomático-Maxilar (33,2%), seguido da Mandíbula (23,7%) e dos Ossos Próprios do Nariz (17%), sendo o gênero masculino o mais acometido por fraturas. Conclusão: a partir desse estudo podemos concluir que os acidentes motociclísticos configuram-se como o principal fator etiológico relacionado as fraturas de face, sendo o gênero, masculino o mais atingido e o tipo de fratura mais prevalente foi a fratura do Complexo Orbito-Zigomático-Maxilar... (AU)

Bone fractures in the maxillofacial region are occurrences that can present themselves as urgent and/or emergencies in the routine of emergency care units and hospitals around the world, especially in locations with high rates of interpersonal violence and traffic violations. A large number of injuries to the face, both in soft and hard tissue, occur due to the enormous exposure and poor protection of this region, which often leads to serious injuries. Objective: This study aims to assess the prevalence of fractures in the maxillofacial complex in a public hospital, based on an epidemiological study, analyzing the factors related to the occurrence of trauma, etiological agent, fracture distribution, gender, and age of patients affected individuals. Methodology: This retrospective cross-sectional study evaluated 268 medical records of patients diagnosed with fractures of the facial bones treated at the Justino Luz Regional Hospital, located in the city of Picos, in the state of Piauí, Brazil, in January 2015 until January 2017, the medical records were analyzed in the medical file sector of the HRJL. Results: the most observed etiological factors were motorcycle accidents, followed by physical aggression and fall from own height, the most common type of fracture was the Orbit-zygomatic-Maxillary Complex (33,2%), followed by the mandible (23,7%) and the nose bonés (17%), being the male gender the most affected by fractures. Conclusion: from this study, we can conclude those motorcycle accidents are the main etiological factor related to facial fractures, with the male gender being the most affected and the most prevalent type of fracture was the fracture of the orbit-zygomatic-maxillary complex... (AU)

Las fracturas óseas en la región maxilofacial son eventos que pueden presentarse como urgentes y/o emergencias en la rutina de las unidades de atención de emergencia y hospitales de todo el mundo, especialmente en lugares con altos índices de violencia interpersonal e infracciones de tránsito. Un gran número de lesiones en la cara, tanto en tejidos blandos como duros, se producen debido a la enorme exposición y escasa protección de esta región, lo que a menudo conduce a lesiones graves. Objetivo: Este estudio tiene como objetivo evaluar la prevalencia de fracturas en el complejo maxilofacial en un hospital público, a partir de un estudio epidemiológico, analizando los factores relacionados con la ocurrencia del trauma, agente etiológico, distribución de la fractura, sexo y edad de los pacientes afectados. Metodología: Este estudio transversal retrospectivo evaluó 268 historias clínicas de pacientes con diagnóstico de fracturas de los huesos faciales atendidos en el Hospital Regional Justino Luz, ubicado en la ciudad de Picos, en el estado de Piauí, Brasil, en enero de 2015 hasta enero de 2017. , las historias clínicas fueron analizadas en el sector de expediente médico del HRJL. Resultados: los factores etiológicos más observados fueron los accidentes de motocicleta, seguido de agresión física y caída de propia altura, el tipo de fractura más común fue el Complejo Órbita-cigomático-Maxilar (33,2%), seguido de la mandíbula (23,7 %) y la nariz bonés (17%), siendo el género masculino el más afectado por las fracturas. Conclusión: de este estudio podemos concluir que los accidentes de motocicleta son el principal factor etiológico relacionado con las fracturas faciales, siendo el género masculino el más afectado y el tipo de fractura más prevalente fue la fractura del complejo orbitario-cigomático-maxilar... (AU)

Murraya paniculata (L.) (Orange Jasmine): Potential Nutraceuticals with Ameliorative Effect in Alloxan-Induced Diabetic Rats.

Orange jasmine, Murraya paniculata (Rutaceae), is a plant from India widely used in folk medicine as antinociceptive, antiinflammatory, and antioxidant. Although oral hypoglycemic agents and insulin are the mainstays of treatment of diabetes mellitus (DM), there is a significant demand for new natural products to reduce the development of diabetic complications. Alloxan-induced diabetic rats were treated for 60 days with a hydroalcoholic extract of M. paniculata (MPE), at doses of 100, 200, and 400 mg/kg. MPE decreased glycemia and also cholesterol and triglyceride levels, starting 1 week after treatments, as compared with the same group before treatments. Glucose values were reduced toward normality after 1 week of treatment. MPE hypoglycemic effects were potentiated by glibenclamide and metformin. MPE also decreased fructosamine and glycated hemoglobin values. MPE reduced diabetes-induced morphological alterations of the kidney, pancreas, and liver. MPE acts similarly to glibenclamide and metformin, and its glucose-lowering action is partly a consequence of ATP-sensitive K+ channel inhibition. MPE may be a potential therapeutic alternative for the treatment of diabetes and its complications. Copyright © 2017 John Wiley & Sons, Ltd.

Increased VEGFR2 and MMP9 protein levels are associated with epithelial dysplasia grading.

The present study aimed to compare levels of VEGFR2 and MMP-9 among control, epithelial dysplasia (ED) and oral squamous cell carcinoma (OSCC) groups. We analyzed 48 patients with oral leukoplakia (OL), 20 patients with OSCC and 21 patients without OL and OSCC. Immunohistochemistry of VEGFR2 and MMP9 were performed and compared among groups. Analysis of tissue immunolocalization of VEGFR2 and MMP-9 assumed non-parametrical distribution and comparison between groups was performed using the Mann-Whitney and Kruskal-Wallis statistical tests. VEGFR2 and MMP9 immunoexpression appeared to correlate with the degree of dysplasia and was observed to increase in lesions with more severe dysplasia as compared to those with lower degrees of dysplasia. Immunoreactivity of MMP-9 was lower in the OL samples compared to the OSCC samples (p = 0.004). We observed no difference in VEGFR2 protein levels between OL and OSCC samples. A positive correlation was found between VEGFR2 and MMP-9 in OL samples (r = +0.452, p = 0.001), however, no correlation was found in OSCC samples (r = -0.042, p = 0.861). In conclusion, the results of the current study suggest that expression of MMP9 and VEGFR2 is associated with ED grading and MMP9 levels are increased in OSCC.

Analysis of the normative conditions of oral health, depression and serotonin-transporter-linked promoter region polymorphisms in an elderly population.

AIM: To investigate the association between depression, the normative conditions of oral health and serotonin-transporter-linked promoter region (5-HTTLPR) polymorphisms in a community-dwelling elderly sample. METHODS: Elderly adults living in a community (n=200) were submitted to an oral clinical examination. Depression was assessed using the 15-item Geriatric Depression Scale. The 5-HTTLPR was analyzed by polymerase chain reaction-restriction fragment length polymorphism. Associations between the biomarkers and clinical covariates were assessed by bivariate and multivariate statistical tests. Statistical significance was set at P<0.05. RESULTS: The prevalence of dental caries, periodontal disease, edentulism and oral mucosal lesions was 51.2%, 20.8%, 58.0% and 21.5%, respectively. The prevalence of depression was 21.5%. A higher frequency of the S allele was recorded in older individuals with depression (P=0.017). The present findings showed no significant association between depression and the normative conditions of oral health. The observed allelic polymorphisms were 44% S and 56% L, respectively. Genotypic variants were 33.5% LL, 45.0% SL and 21.5% SS. Individuals carrying the SS genotype showed a significantly high prevalence of periodontal disease (prevalence ratio=3.077, CI 95%=1.008-9.393, P=0.048). CONCLUSION: The present findings suggest that depressive community-dwelling older individuals did not show any association with alterations of oral health normative conditions. However, the SS genotypic polymorphic variant of 5-HTTLPR seems to contribute to the occurrence of periodontal disease in the elderly.

DNA repair gene excision repair cross complementing-group 1 (ERCC1) in head and neck squamous cell carcinoma: analysis of methylation and polymorphism (G19007A), protein expression and association with epidemiological and clinicopathological factors.

AIMS: To evaluate the associations of excision repair cross complementing-group 1 (ERCC1) (DNA repair protein) (G19007A) polymorphism, methylation and immunohistochemical expression with epidemiological and clinicopathological factors and with overall survival in head and neck squamous cell carcinoma (HNSCC) patients. METHODS AND RESULTS: The study group comprised 84 patients with HNSCC who underwent surgery and adjuvant radiotherapy without chemotherapy. Bivariate and multivariate analyses were used. The allele A genotype variant was observed in 79.8% of the samples, GG in 20.2%, GA in 28.6% and AA in 51.2%. Individuals aged more than 45 years had a higher prevalence of the allelic A variant and a high (83.3%) immunohistochemical expression of ERCC1 protein [odds ratio (OR) = 4.86, 95% confidence interval (CI): 1.2-19.7, P = 0.027], which was also high in patients with advanced stage (OR=5.04, 95% CI: 1.07-23.7, P = 0.041). Methylated status was found in 51.2% of the samples, and was higher in patients who did not present distant metastasis (OR = 6.67, 95% CI: 1.40-33.33, P = 0.019) and in patients with advanced stage (OR = 5.04, 95% CI: 1.07-23.7, P = 0.041). At 2 and 5 years, overall survival was 55% and 36%, respectively (median = 30 months). CONCLUSION: Our findings may reflect a high rate of DNA repair due to frequent tissue injury during the lifetime of these individuals, and also more advanced disease presentation in this population with worse prognosis.

Association of -308 TNF-α promoter polymorphism with clinical aggressiveness in patients with head and neck squamous cell carcinoma.

Genetic polymorphisms in the promoter region of the tumour necrosis factor-α (TNF-α) gene are involved in the regulation of the expression levels of its cytokine. Besides, these polymorphisms have been associated with the clinical behaviour of cancer. We investigated the -308 promoter region polymorphisms of the TNF-α gene and its association with the clinicopathological factors of a head and neck squamous cell carcinoma (HNSCC) sample. Furthermore, we analysed the impact of all the variables on the overall survival of patients. A sample of HNSCC (n=89) was evaluated. Clinicopathological factors and overall survival data were gathered. The TNF-α gene was analysed by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Data analyses were performed by using bivariate and multivariate statistical tests. Significance was set at p<0.05. HNSCC subjects carrying the A allele (GA/AA) exhibited associations with poor performance status (OR=2.82, p=0.039), lesions located on posterior areas (OR=4.02, p=0.002), and large-size tumours (OR=2.91, p=0.015). Subjects carrying only AA genotype exhibited association with poor performance status (OR=6.667, p=0.007). A worse overall survival was noted in subjects with large tumours (OR=4.87, p=0.005) and locoregional metastatic disease (OR=2.50, p=0.018). Our data suggests that the presence of the A allele/AA haplotype in HNSCC individuals might contribute to the higher clinical aggressiveness of malignant disease.

Effect of age on the association between p16CDKN2A methylation and DNMT3B polymorphism in head and neck carcinoma and patient survival.

De novo DNA methylation is a relevant epigenetic mechanism, which represses gene transcription and commonly inactivates tumor suppressor genes in carcinogenesis. A single nucleotide polymorphism of DNMT3B, C46359T (-149C-->T) was reported to modulate individual's susceptibility to cancer. We investigated the role of this polymorphic variant regarding the methylation status of the p16CDKN2A gene in young and older patients with head and neck squamous cell carcinoma (HNCC) matched by the TNM staging system, together with its impact on patients survival. The results showed that the presence of the allele T of the polymorphism DNMT3B (-149C-->T) was associated with advanced TNM staging and smoking habit, but no association was found between this polymorphisms and DNMT3B immunostaining. While p16CDKN2A methylation was significantly associated with smoking habit in older patients, this parameter was associated with family history of cancer in young patients. Moreover, in older patients the absence of p16CDKN2A promoter methylation had a negative impact on survival. In conclusion, nucleotide polymorphism of DNMT3B is not associated with methylation of p16CDKN2A gene in HNSCC. The association of p16CDKN2A gene methylation with smoking, family history of cancer and survival is dependent on age.